Colin Montgomerie and Jose Maria Olazabal were among those to miss out in the Open Championship qualifier at Sunningdale, but England's Sam Walker will finally make his debut at Royal Lytham next month after 10 previous attempts.
Montgomerie, who took two strokes too many in a qualifying event for the US Open four weeks ago, came even closer this time but was left needing to hole a bunker shot on the final hole to go into a play-off.
The shot had the length but finished six feet wide, and so the 49-year-old was able to add only a level-par 70 on the Old Course to his morning three-under 66 round the adjoining New.
Olazabal, the Scot's successor as Ryder Cup captain, was on track to make it when he reached five under after 14 holes, but fell back to one under with rounds of 67 and 71.
Walker, who made his first try to qualify 14 years ago when he was 20, finished second to compatriot James Morrison as the 96-strong field battled for 10 spots. Morrison, another who will be making his Open debut, finished eight under after rounds of 63 and 68.
Sparkling though Morrison's first round was, it was bettered by one in the afternoon by Welshman Jamie Donaldson as he made it through with only a single shot to spare. That 62 equalled the course record which Montgomerie himself matched two years ago.
For Donaldson it will be a second Open, while England's Matthew Baldwin will play his first a month after qualifying for a US Open debut. He too finished five under, as did compatriot Richard Finch, Spain's Alejandro Canizares and Australian Marcus Fraser.
However, Finch's fellow Yorkshireman Danny Willett, who beat Fraser in a four-hole play-off for the BMW International Open in Cologne on Sunday, missed out by four shots. After a 9.30pm flight back from Germany, Willett had "a couple of beers" to celebrate his first European Tour victory and did not get to bed until 2am.
Others to fail included Tom Lewis, who led after the opening day at Sandwich last July and with his 65 achieved the lowest round ever by an amateur in The Open, Italian teenager Matteo Manassero and Swede Niclas Fasth, runner-up to David Duval on Lytham's last staging of the championship in 2001.
Willett could still qualify through a mini money list which ends this week at the Irish Open. He is just over £100 behind South African Richard Sterne, who missed out himself by one stroke. Five players - English pair Lee Slattery and Oliver Fisher, Scot Alastair Forsyth, Swede Oscar Floren and Dane Thorbjorn Olesen - were involved in the play-off for the last two places on offer